The Datta Lab investigates the fundamental biological and bioprocess factors that govern recombinant protein biosynthesis, folding, stability, and functional activity. Our research seeks to understand how cellular systems produce complex proteins and how host-cell physiology and cultivation conditions influence protein quality and performance.
Using Escherichia coli as a model microbial host and CRISPR-associated protein 9 (Cas9) as a model multidomain protein, we study the mechanisms that limit the production of active recombinant proteins. High-level protein expression often imposes stress on cellular quality-control systems, leading to protein misfolding, aggregation, degradation, and reduced biological activity. Understanding these processes is essential for advancing both basic biological knowledge and biomanufacturing technologies.
Our work integrates molecular biology, microbial biotechnology, protein science, and bioprocess engineering to identify the cellular and environmental factors that influence recombinant protein outcomes. By linking molecular mechanisms with bioprocess performance, we aim to establish general principles that govern protein production across biological systems.
A complementary area of research focuses on the development of cell-based and biomolecular interaction assays to investigate biological activity, potency, and structure-function relationships of recombinant biomolecules.
🧬 Research Themes
- Recombinant protein biosynthesis and quality control
- Bioprocess and cultivation effects on recombinant protein quality
- Determinants of recombinant protein stability
The laboratory provides hands-on research experiences for undergraduate and graduate students, integrating training in molecular biology, microbial biotechnology, protein analysis, and bioprocessing with hypothesis-driven research.
Publications → View our research publications and scholarly contributions. Link to Publications
Student Research and Training
The laboratory provides hands-on research opportunities for undergraduate and graduate students. Trainees participate in hypothesis-driven research while developing skills in molecular biology, microbial biotechnology, protein analysis, and bioprocess engineering. Through these experiences, students learn how fundamental biological processes can be investigated and applied to address challenges in biotechnology and biomanufacturing.
Collaborate With Us
We actively seek collaborations with academic institutions, industry partners, and other research groups. We believe that working together can accelerate discoveries and translate them into real-world solutions faster.
Let's explore how our combined efforts can lead to groundbreaking innovations.
Please reach out to us at [email protected] to discuss potential collaborative opportunities.